The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 21, 2017
Filed:
Nov. 26, 2014
Applicant:
Burnham Institute for Medical Research, La Jolla, CA (US);
Inventors:
Alexey Terskikh, San Diego, CA (US);
Ruchi Bajpai, Redwood City, CA (US);
Assignee:
BURNHAM INSTITUTE FOR MEDICAL RESEARCH, San Diego, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C12N 5/0735 (2010.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); G01N 33/5073 (2013.01); G01N 33/56966 (2013.01); C12N 2509/00 (2013.01);
Abstract
We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.