The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9588125 B1

PDF
Full Text
Expired
Date of Patent:
Mar. 07, 2017

Filed:

Mar. 14, 2014
Applicant:

Shimadzu Corporation, Kyoto-shi, Kyoto, JP;

Inventors:

Akiyasu Yoshizawa, Kyoto, JP;

Takayuki Yoshimori, Nerima-ku, JP;

Yuki Ohta, Kawasaki, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/37 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); C12Q 1/37 (2013.01);
Abstract

A peptide is cleaved at various bonding sites into oligopeptides or similar fragments by digestion using proteinase K (S). The obtained fragments are separated according to their kinds by reversed-phase chromatography and fractionated (S), and each fragment is subjected to mass spectrometry to determine its mass (S). For each peptide fragment, an amino-acid composition is calculated from the measured mass, and amino-acid sequence candidates are deduced from that composition. The amino-acid sequence candidates of the other peptide fragments are searched for a fragment having an overlapping portion available for combining two peptide fragments to obtain amino-acid sequence candidates of the original peptide (S). The masses of the amino-acid sequence candidates are compared with a measured mass derived from a result of mass spectrometry of the original peptide to select a correct sequence (Sand S).


Find Patent Forward Citations

Yoshizawa, Akiyasu. (2017). Method and system for analyzing protein or peptide (U.S. Patent No. 9588125). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9588125

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