The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9588105 B1

PDF
Full Text
Expired
Date of Patent:
Mar. 07, 2017

Filed:

Mar. 14, 2014
Applicant:

The United States of America, As Represented BY the Secretary of the Air Force, Washington, DC (US);

Inventors:

Saber M. Hussain, Beavercreek, OH (US);

Christin M. Grabinski, Centerville, OH (US);

James E. Grabinski, Lowell, IN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/00 (2006.01); G01N 33/50 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/36 (2006.01); C12M 1/32 (2006.01); C12M 1/42 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5008 (2013.01); C12M 23/12 (2013.01); C12M 25/00 (2013.01); C12M 29/00 (2013.01); C12M 35/02 (2013.01); C12M 41/48 (2013.01); C12M 47/00 (2013.01);
Abstract

A single- or multi-well chamber assembly comprising nanoparticle exposure chambers with a plurality of wells designed to hold porous membrane inserts, with cell culture media being maintained on the basal (lower) surface to generate the air-liquid interface. The wells may accommodate a variety of sizes of cell culture inserts and may be arranged radially. The chamber assembly comprises an integrated heating-humidifying mechanism so that the internal chamber environment may be heated to 37° C. and maintained at humid conditions to allow for maintenance of cell cultures. The presently disclosed apparatus may be used to investigate the toxicity of airborne nanoparticles to cells or tissue models grown at the air-liquid interface. Aerosolized nanomaterials are drawn into a multi-well chamber assembly and deposited via electrostatic deposition onto cells grown at the air-liquid interface.


Find Patent Forward Citations

Hussain, Saber. (2017). Portable in vitro multi-well chamber for exposing airborne nanomaterials at the air-liquid interface using electrostatic deposition (U.S. Patent No. 9588105). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9588105

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