The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 07, 2017

Filed:

Sep. 07, 2012
Applicants:

Yoshihide Hayashizaki, Wako, JP;

Yasumasa Kimura, Wako, JP;

Kengo Usui, Wako, JP;

Yuki Tanaka, Wako, JP;

Yuki Kawai, Yokohama, JP;

Inventors:

Yoshihide Hayashizaki, Wako, JP;

Yasumasa Kimura, Wako, JP;

Kengo Usui, Wako, JP;

Yuki Tanaka, Wako, JP;

Yuki Kawai, Yokohama, JP;

Assignee:

Kabushiki Kaisha DNAFORM, Kanagawa, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C07H 21/04 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6853 (2013.01);
Abstract

The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A′) that can hybridize to a sequence (A) on the 3′ side of the target nucleic acid sequence. The second primer 1R includes, on the 3′ side thereof, a sequence (B′) that can hybridize to a sequence (B) on the 3′ side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5′ sides thereof, sequences (C) that are substantially identical to each other.


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