The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2017
Filed:
Apr. 07, 2015
Applicant:
New England Biolabs, Inc., Ipswich, MA (US);
Inventors:
Nathan Tanner, Peabody, MA (US);
Yinhua Zhang, North Reading, MA (US);
Thomas C. Evans, Topsfield, MA (US);
Assignee:
New England BioLabs, Inc., Ipswich, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01); G01N 21/80 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/6844 (2013.01); G01N 21/6486 (2013.01); G01N 21/80 (2013.01);
Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.