The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 21, 2017

Filed:

Aug. 21, 2014
Applicant:

Olympus Corporation, Tokyo, JP;

Inventors:

Kazutaka Nishikawa, Tokyo, JP;

Takuya Hanashi, Tokyo, JP;

Assignee:

OLYMPUS CORPORATION, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/64 (2006.01); G01N 21/76 (2006.01); G01N 33/52 (2006.01); G01N 33/53 (2006.01); G02B 21/00 (2006.01); G01N 33/543 (2006.01); G01N 15/14 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5308 (2013.01); G01N 15/1434 (2013.01); G01N 21/6486 (2013.01); G01N 21/76 (2013.01); G01N 33/52 (2013.01); G01N 33/54313 (2013.01); G02B 21/00 (2013.01); G02B 21/0076 (2013.01); G01N 2201/12 (2013.01); Y10T 436/143333 (2015.01);
Abstract

Provided is a method for detecting a target particle that is a method for detecting a non-luminescent target particle dispersed and randomly moving in a sample solution using an optical system of a confocal microscope or multi-photon microscope, having: (a) preparing a sample solution containing target particles, and labeling particles of which the average outer diameter is less than 15% of the diameter of a photodetection region of the optical system, binding two or more molecules of the labeling particles per molecule of the target particles in the sample solution, and forming a non-luminescent complex of which the outer diameter is 15% or more of the diameter of the photodetection region; and, (b) calculating the number of molecules of the complex in the sample solution prepared in the (a) using an inverse scanning molecule counting method.


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