The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 21, 2017

Filed:

Jul. 31, 2014
Applicant:

Enzo Life Sciences, Inc., New York, NY (US);

Inventors:

Maciej Szczepanik, Mount Sinai, NY (US);

Irina V. Lebedeva, Bronx, NY (US);

Yuejun Xiang, Hollis Hills, NY (US);

Praveen Pande, Holbrook, NY (US);

Wayne Forrest Patton, Dix Hills, NY (US);

Assignee:

Enzo Life Sciences, Inc., New York, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/26 (2006.01); C12Q 1/48 (2006.01); C09K 11/06 (2006.01); C12Q 1/37 (2006.01); C12Q 1/44 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/485 (2013.01); C09K 11/06 (2013.01); C12Q 1/26 (2013.01); C12Q 1/37 (2013.01); C12Q 1/44 (2013.01); G01N 2333/902 (2013.01); G01N 2500/00 (2013.01); Y10T 436/18 (2015.01);
Abstract

Provided is a compound comprising the structure:(SIG)-(SI-MOD). In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.


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