The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 21, 2017

Filed:

Apr. 14, 2014
Applicant:

Phyton Holdings, Llc, San Antonio, TX (US);

Inventors:

Kazuhito Fujiyama, Osaka, JP;

Tatsuji Seki, Osaka, JP;

Toshiomi Yoshida, Osaka, JP;

Assignee:

Phyton Holdings, LLC, Fort Worth, TX (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/82 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C12N 15/8257 (2013.01);
Abstract

A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell. The method further includes isolating the glycoprotein from the culture medium and contacting the isolated glycoprotein with a sialic acid transferase to add a sialic acid residue attached to the galactose residue in the N-glycan.


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