The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 21, 2017
Filed:
May. 23, 2012
Applicant:
Hiroaki Udagawa, Chiba, JP;
Inventor:
Hiroaki Udagawa, Chiba, JP;
Assignee:
Novozymes A/S, Bagsvaerd, DK;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/02 (2006.01); C12N 15/80 (2006.01);
U.S. Cl.
CPC ...
C12N 15/80 (2013.01); C12P 21/02 (2013.01);
Abstract
The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.