The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 21, 2017

Filed:

Nov. 30, 2006
Applicants:

Kenton C. Hasson, Gaithersburg, MD (US);

Gregory A. Dale, Gaithersburg, MD (US);

Inventors:

Kenton C. Hasson, Gaithersburg, MD (US);

Gregory A. Dale, Gaithersburg, MD (US);

Assignee:

Canon U.S. Life Sciences, Inc., Rockville, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 7/00 (2006.01); C12Q 1/68 (2006.01); G01N 35/08 (2006.01); B01L 3/00 (2006.01);
U.S. Cl.
CPC ...
B01L 7/52 (2013.01); C12Q 1/686 (2013.01); G01N 35/08 (2013.01); B01L 3/5027 (2013.01); B01L 2200/143 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0487 (2013.01); Y10T 436/115831 (2015.01); Y10T 436/117497 (2015.01);
Abstract

The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. A method according to one embodiment of the invention may include the steps of: forcing a sample of a solution containing real-time PCR reagents to move though a channel; and while the sample is moving through an analysis region of the channel, performing the steps of: (a) cycling the temperature of the sample until the occurrence of a predetermined event; (b) after performing step (a), causing the sample's temperature to gradually increase from a first temperature to a second temperature; and (c) while the step of gradually increasing the sample's temperature is performed, using an image sensor to monitor emissions from the sample.


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