The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 14, 2017
Filed:
Sep. 29, 2014
The United States of America, As Represented BY the Secretary of Agriculture, Washington, DC (US);
David M. Donovan, Baltimore, MD (US);
Igor V. Abaev, Obolensk, UA;
The United States of America, as Represented by the Secretary of Agriculture, Washington, DC (US);
Abstract
Increases in antibiotic resistant strains ofhave elicited efforts to develop novel antimicrobials. One potential treatment includes lytic enzymes produced by staphylococcal bacteriophage during the lytic cycle. The phage Twort endolysin (PlyTW) harbors three domains, a CHAP endopeptidase, an amidase-2 domain, and a SH3b-5 cell wall binding domain. The CHAP domain alone is necessary and sufficient for lysis of live; the amidase-2 domain alone is insufficient. Loss of the SH3b cell wall binding domain results in a 10 fold reduction of enzymatic activity in turbidity reduction and plate lysis assays compared to the full length protein. Deletion of the amidase-2 domain resulted in a protein (PlyTW Δ172-373) with lytic activity that exceeded the activity of the full length construct in both assays. Addition of Caenhanced activity in turbidity reduction assays. Chelation by the addition of EDTA or zinc inhibited the activity of all PlyTW constructs.