The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 2017

Filed:

Aug. 11, 2011
Applicants:

Alexei Kirkin, Copenhagen, DK;

Karine Djandjougazian, Copenhagen, DK;

Martin Roland Jensen, Holte, DK;

Inventors:

Alexei Kirkin, Copenhagen, DK;

Karine Djandjougazian, Copenhagen, DK;

Martin Roland Jensen, Holte, DK;

Assignee:

Cytovac A/S, Horsholm, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0784 (2010.01); C12N 5/0783 (2010.01); A61K 35/15 (2015.01); A61K 35/17 (2015.01); A61K 39/00 (2006.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0639 (2013.01); A61K 35/15 (2013.01); A61K 35/17 (2013.01); A61K 39/0011 (2013.01); C12N 5/0638 (2013.01); A61K 2035/124 (2013.01); A61K 2039/5154 (2013.01); A61K 2039/5158 (2013.01); C12N 2500/90 (2013.01); C12N 2501/02 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/25 (2013.01); C12N 2501/91 (2013.01); C12N 2506/115 (2013.01); C12N 2533/70 (2013.01); C12N 2533/90 (2013.01);
Abstract

The present invention relates to compositions and methods for producing dendritic cells and particularly to compositions and methods for producing immature dendritic cells that are immunocompetent. We describe a method of producing dendritic cells by cultivation of monocytes, characterised by at least one of: pre-treatment of a tissue culture surface with at least one of: a substantially plasma-free and serum-free pre-treatment medium, a pre-treatment medium comprising heparin, and a pre-treatment medium comprising a protein solution; adsorption of monocytes using at least one of: a substantially plasma-free and serum-free adsorption medium; cultivation of monocytes using a substantially plasma-free and serum-free cultivation medium. We also describe compositions including the dentritic cells and uses of the dentritic cells.


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