The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 31, 2017
Filed:
Dec. 12, 2012
Universite Joseph Fourier-grenoble 1, St. Martin d'Hères, FR;
Centre Hospitalier Universitaire Grenoble, La Tronche, FR;
Audrey Le Gouellec, Uriage, FR;
Bertrand Toussaint, Saint Egreve, FR;
Benoît Polack, Saint Martin le Vinoux, FR;
UNIVERSITE GRENOBLE ALPES, Saint Martin d'Hères, FR;
CENTRE HOSPITALIER UNIVERSITAIRE GRENOBLE, La Tronche, FR;
Abstract
A process for obtaining an adapted strain ofincludes the following steps: deleting the genes ExoS, ExoT, aroA and IasI in an initialstrain cultivated in a LB medium; progressively cultivating this strain in a chemically defined medium based on a glucose minimal medium supplemented with magnesium and calcium; wherein the adapted strain presents the same toxicity and secretion capacities than the initial strain, and its doubling time when cultivated in the chemically defined medium is less than 60 minutes. An adapted strain is furthermore treated to become 'killed but metabolically active'.