The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 31, 2017
Filed:
Jul. 03, 2012
Joseph Nti-gyabaah, West Greenwich, RI (US);
Ijeoma Ikechukwu, Carteret, NJ (US);
Matthew Pettroff, Secaucus, NJ (US);
Christine Cho Lee, Cohoes, NY (US);
Joseph Nti-Gyabaah, West Greenwich, RI (US);
Ijeoma Ikechukwu, Carteret, NJ (US);
Matthew Pettroff, Secaucus, NJ (US);
Christine Cho Lee, Cohoes, NY (US);
Merck Sharp & Dohme Corp., Rahway, NJ (US);
Abstract
The invention relates generally to methods for purifying a Fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing TNFR:Fc for human administration which comprises an optimized Protein A affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode.