The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 31, 2017
Filed:
Aug. 02, 2012
Andrei P. Pomerantsev, Bethesda, MD (US);
Stephen H. Leppla, Bethesda, MD (US);
Andrei P. Pomerantsev, Bethesda, MD (US);
Stephen H. Leppla, Bethesda, MD (US);
Abstract
The invention relates to a() in which more than one secreted protease is inactivated by genetic modification. Such a protease-deficienthas an improved ability to produce recombinant secreted proteins compared to other bacteria, particularly other. Improvements include production of intact (i.e., mature full-length) proteins, often at high yield. The disclosure provides athat comprises a genetic modification that inactivates a protease of the M4 family of metalloproteases and a genetic modification that inactivates a protease of the M6 family of metalloproteases. Also provided is a modifiedcomprising such genetic modification transformed with a recombinant molecule encoding a product, as well as methods to prepare and use such