The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 17, 2017

Filed:

Jan. 22, 2015
Applicant:

Dh Technologies Development Pte. Ltd., Singapore, SG;

Inventors:

Brian L. Williamson, Ashland, MA (US);

Subhasish Purkayastha, Acton, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 24/00 (2006.01); C12Q 1/26 (2006.01); C12N 9/02 (2006.01); G01N 33/68 (2006.01); G01N 33/50 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/26 (2013.01); C12N 9/0077 (2013.01); G01N 33/502 (2013.01); G01N 33/5023 (2013.01); G01N 33/5067 (2013.01); G01N 33/6848 (2013.01); G01N 30/7233 (2013.01); G01N 2030/8831 (2013.01); G01N 2333/90245 (2013.01); G01N 2500/04 (2013.01); G01N 2500/10 (2013.01); G01N 2500/20 (2013.01); G01N 2560/00 (2013.01); Y10T 436/24 (2015.01);
Abstract

A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.


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