The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 17, 2017

Filed:

May. 31, 2011
Applicants:

How Tseng, Taipei, TW;

Jeng-kuen Tsai, Taipei, TW;

Keng-liang Ou, Taipei, TW;

Po-nien Chen, Taipei, TW;

Inventors:

How Tseng, Taipei, TW;

Jeng-Kuen Tsai, Taipei, TW;

Keng-Liang Ou, Taipei, TW;

Po-Nien Chen, Taipei, TW;

Assignee:
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/26 (2006.01); C12N 5/00 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
C12M 25/00 (2013.01); C12M 25/06 (2013.01); C12M 33/00 (2013.01); C12N 5/0068 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01);
Abstract

The invention creates a support for cell culture and cell sheet detachment which has a substrate, whose surface is coated with a conjugate having a disulfide bond-containing amino acid as a spacer and a biopolymer enhancing cell attachment, migration or differentation. Unexpectedly, after being seeded on the support, the cells grow to form one or more layers of cell sheets and the cell sheets can be easily detached from the support by adding a reductant to cleave the disulfide bond. Accordingly, the invention provides a simple and non-toxic method for detachment of cell sheets.


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