The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2017
Filed:
May. 24, 2013
Applicant:
California Institute of Technology, Pasadena, CA (US);
Inventors:
Assignee:
California Institute of Technology, Pasadena, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 15/86 (2006.01); C12N 15/90 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C12N 15/86 (2013.01); G01N 33/582 (2013.01); G01N 33/68 (2013.01); C07K 2319/00 (2013.01); C12N 15/907 (2013.01); C12N 2740/15043 (2013.01);
Abstract
Some embodiments herein provide compositions and methods for expressing secreted and cell-surface-bound polypeptides in a single cell. In some embodiments, secreted and cell-surface polypeptide are produced from a single polynucleotide. The polynucleotide can comprise a sequence (or sequence encoding a polypeptide) that mediates separation of a membrane anchor from the polypeptide. In some embodiments, a desired ratio of secreted to surface-bound polypeptide is obtained by selecting a sequence that mediates a desired level of separation of the membrane anchor from the polypeptide.