The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 2017
Filed:
Apr. 22, 2014
Alan J. Russell, Gibsonia, PA (US);
Richard R. Koepsel, Pittsburgh, PA (US);
Chad Cummings, Pittsburgh, PA (US);
Hironobu Murata, Pittsburgh, PA (US);
Alan J. Russell, Gibsonia, PA (US);
Richard R. Koepsel, Pittsburgh, PA (US);
Chad Cummings, Pittsburgh, PA (US);
Hironobu Murata, Pittsburgh, PA (US);
Carnegie Mellon University, Pittsburgh, PA (US);
University of Pittsburgh, Pittsburgh, PA (US);
Abstract
Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.