The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 03, 2017

Filed:

Jun. 18, 2012
Applicants:

Carol V. Robinson, Oxford, GB;

Sheila Wang, Oxford, GB;

Nelson P. Barrera, Oxford, GB;

Inventors:

Carol V. Robinson, Oxford, GB;

Sheila Wang, Oxford, GB;

Nelson P. Barrera, Oxford, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 24/00 (2006.01); H01J 49/00 (2006.01); G01N 33/68 (2006.01); G01N 33/94 (2006.01); H01J 49/02 (2006.01); B01D 15/38 (2006.01);
U.S. Cl.
CPC ...
H01J 49/0027 (2013.01); G01N 33/6848 (2013.01); G01N 33/94 (2013.01); H01J 49/02 (2013.01); B01D 15/3842 (2013.01); G01N 2333/705 (2013.01);
Abstract

According to the present invention, there is provided a method of detecting a complex comprising a membrane protein bound to a therapeutic agent by mass spectrometry. The method comprises: (a) providing a solution comprising a detergent micelle in which said complex is contained; (b) providing a mass spectrometer comprising a nanoelectrospray ionization source, a mass analyzer and a detector; (c) vaporizing the solution using the nanoelectrospray ionization source under conditions such that the complex is released from the micelle; (d) ionizing the complex; (e) resolving the ionized complex using the mass analyzer; and (f) detecting the resolved complex using the detector. Also provided is a solution comprising a detergent micelle in which a complex is contained, wherein the complex comprises a membrane protein bound to a therapeutic agent.


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