The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 27, 2016

Filed:

Oct. 13, 2015
Applicant:

King Saud University, Riyadh, SA;

Inventors:

Md. Ashrafuzzaman, Riyadh, SA;

Chih-Yuan Tseng, Edmonton, CA;

Assignee:

KING SAUD UNIVERSITY, Riyadh, SA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/92 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G01N 33/92 (2013.01); G01N 33/5014 (2013.01);
Abstract

The method for direct detection of lipid binding agents in membrane includes dissolving a lipid in an organic solvent in a container; evaporating off the organic solvent to create a lipid film on the inside wall of the container; adding a buffer solution; adding a known volume of a membrane active agent to the buffer solution; incubating the solution in the dark for a period of time; removing the buffer solution from the container to provide a solution of sample A; washing the container with a buffer solution; adding an organic solvent while stirring the container to dissolve the lipid film to create a homogenous solution B; and measuring the absorbance of the samples A and B by absorption spectroscopy. The method may further include developing a universal probability function to test and quantify a membrane-based cytotoxicity of general drug candidates.


Find Patent Forward Citations

Loading…