The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 20, 2016
Filed:
Jan. 13, 2014
Applicant:
Unitaq Bio, Los Altos, CA (US);
Inventors:
Eugene Spier, Los Altos, CA (US);
Karl Guegler, Menlo Park, CA (US);
Assignee:
Uni Taq Bio, Los Altos, CA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12Q 1/68 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); C12Q 1/686 (2013.01); C12Q 2525/15 (2013.01); C12Q 2537/143 (2013.01); C12Q 2537/163 (2013.01);
Abstract
Provided herein are methods and compositions for performing PCR with primers with blocked 3'-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations.