The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 20, 2016

Filed:

Jul. 02, 2014
Applicant:

The Catholic University of America, Washington, DC (US);

Inventor:

Venigalla B. Rao, Silver Spring, MD (US);

Assignee:

The Catholic University of America, Washington, DC (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/86 (2006.01); C12N 15/87 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); C12N 15/88 (2006.01); A61K 47/48 (2006.01); A61K 31/7088 (2006.01); A61K 31/711 (2006.01); A61K 48/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/86 (2013.01); A61K 31/7088 (2013.01); A61K 31/711 (2013.01); A61K 47/48776 (2013.01); C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 15/87 (2013.01); C12N 15/88 (2013.01); A61K 48/00 (2013.01); C12N 2795/10042 (2013.01); C12N 2795/10122 (2013.01); C12N 2795/10142 (2013.01); C12N 2795/10152 (2013.01);
Abstract

Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging. An aspect of the invention relates to the phage T4 packaging machine being highly promiscuous, translocating DNA into finished phage heads as well as into proheads. Single motors can force exogenous DNA into phage heads at the same rate as into proheads and phage heads undergo repeated initiations, packaging multiple DNA molecules into the same head. This shows that the phage DNA packaging machine has unusual conformational plasticity, powering DNA into an apparently passive capsid receptacle, including the highly stable virus shell, until it is full. These features allow for the design of a novel class of nanocapsid delivery vehicles.


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