The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 20, 2016
Filed:
Sep. 09, 2010
Anne Schönbrunn, Berlin, DE;
Dan Robin Miller, Berlin, DE;
Siegfried Kohler, Berlin, DE;
Marco Frentsch, Berlin, DE;
Andreas Thiel, Berlin, DE;
Anne Schönbrunn, Berlin, DE;
Dan Robin Miller, Berlin, DE;
Siegfried Kohler, Berlin, DE;
Marco Frentsch, Berlin, DE;
Andreas Thiel, Berlin, DE;
MILTENYI BIOTEC GMBH, Bergisch Gladbach, DE;
Abstract
The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, 'latent TGF-beta (LAP)', GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles.