The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 13, 2016

Filed:

Feb. 26, 2013
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Yi-Zhen Hu, Eugene, OR (US);

Aimei Chen, Eugene, OR (US);

Hee Chol Kang, Eugene, OR (US);

Kyle Gee, Springfield, OR (US);

Bhaskar Mandavilli, Eugene, OR (US);

Assignee:

LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07D 215/00 (2006.01); G01N 33/52 (2006.01); C09B 23/02 (2006.01); C09B 23/04 (2006.01); C09B 23/06 (2006.01); G01N 33/84 (2006.01); C07D 417/06 (2006.01);
U.S. Cl.
CPC ...
G01N 33/52 (2013.01); C07D 417/06 (2013.01); C09B 23/02 (2013.01); C09B 23/04 (2013.01); C09B 23/06 (2013.01); G01N 33/84 (2013.01);
Abstract

Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.


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