The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 13, 2016
Filed:
Jun. 28, 2012
Yoshihide Hayashizaki, Yokohama, JP;
Kengo Usui, Yokohama, JP;
Saori Goda, Yokohama, JP;
Kazuhito Nomura, Yokohama, JP;
Yuki Kawai, Yokohama, JP;
Yoshihide Hayashizaki, Yokohama, JP;
Kengo Usui, Yokohama, JP;
Saori Goda, Yokohama, JP;
Kazuhito Nomura, Yokohama, JP;
Yuki Kawai, Yokohama, JP;
KABUSHIKI KAISHA DNAFORM, Kanagawa, JP;
Abstract
The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.