The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 13, 2016
Filed:
Dec. 26, 2014
Jian Chen, Wuxi, CN;
Jingwen Zhou, Wuxi, CN;
Hongwei Guo, Wuxi, CN;
Yongkun LV, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jian Chen, Wuxi, CN;
Jingwen Zhou, Wuxi, CN;
Hongwei Guo, Wuxi, CN;
Yongkun Lv, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jiangnan University, Wuxi, JS, CN;
Abstract
The present invention provides a method for decreasing pyruvate catabolism and increasing the accumulation of pyruvate in microbes. By overexpressing wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutants which have mutations at conservative active sites, the present invention provide a method to decrease overall activity of pyruvate dehydrogenase complex and pyruvate catabolism, and thus increase the accumulation of extracellular pyruvate without killing the pyruvate-producing microbes. Overexpressing dihydrolipoamide acetyltransferase mutants is an effective way to increase pyruvate accumulation.