The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 13, 2016
Filed:
Feb. 07, 2014
Applicant:
Merck Sharp & Dohme Corp., Rahway, NJ (US);
Inventor:
Byung-Kwon Choi, Norwich, VT (US);
Assignee:
Merck Sharp & Dohme Corp., Rahway, NJ (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/15 (2006.01); C07K 14/44 (2006.01); C07K 14/39 (2006.01); C12P 21/00 (2006.01); C12N 9/10 (2006.01); C07K 14/62 (2006.01); C07K 16/28 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/44 (2013.01); C07K 14/39 (2013.01); C07K 14/62 (2013.01); C07K 16/2863 (2013.01); C12N 9/1051 (2013.01); C12P 21/005 (2013.01); A61K 38/00 (2013.01); C07K 2317/14 (2013.01); C07K 2317/41 (2013.01); Y02P 20/52 (2015.11);
Abstract
Methods are disclosed for increasing the yield and N-glycosylation site occupancy of paucimannose or complex N-glycans of recombinant glycoproteins produced in a recombinant host cell lacking dolichyl-P-Man:ManGlcNAc-PP-dolichyl alpha-1,3 mannosyltransferase (Alg3p) activity. In particular, the present invention provides recombinant host cells for producing recombinant glycoproteins that comprise a disruption of the expression of an OS-9 family gene in the host cell and which overexpress one or moreSTT3 proteins.