The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 13, 2016

Filed:

Apr. 07, 2014
Applicant:

Kuros Biosciences Ag, Schlieren, CH;

Inventors:

Marcel Emmerling, Schlieren, CH;

Frank Hennecke, Dietlikon, CH;

Holger Pfründer, Zürich, CH;

Martin Rhiel, Bonstetten, CH;

Philipp Steiner, Schlieren, CH;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 7/01 (2006.01); C12N 15/11 (2006.01); A61K 35/76 (2015.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); A61K 38/00 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); C12N 7/00 (2013.01); A61K 2039/5256 (2013.01); A61K 2039/5258 (2013.01); C12N 2795/00023 (2013.01); C12N 2795/00051 (2013.01); C12N 2795/10051 (2013.01); C12N 2795/10061 (2013.01); C12N 2795/18022 (2013.01); C12N 2795/18052 (2013.01);
Abstract

This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.


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