The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 06, 2016

Filed:

Nov. 08, 2012
Applicant:

University of Washington Through Its Center for Commercialization, Seattle, WA (US);

Inventors:

Michael H. Gelb, Seattle, WA (US);

Frantisek Turecek, Seattle, WA (US);

Zdenek Spacil, Seattle, WA (US);

C. Ronald Scott, Seattle, WA (US);

Mariana Natali Barcenas Rodriguez, Seattle, WA (US);

Assignee:

University of Washington, Seattle, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/34 (2006.01); G01N 33/573 (2006.01); C12Q 1/44 (2006.01); G01N 30/72 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/34 (2013.01); C12Q 1/44 (2013.01); G01N 33/573 (2013.01); G01N 30/7233 (2013.01);
Abstract

Methods and liquid compositions for assaying the activity of one or more lysosomal enzymes in a sample are provided. In some embodiments, the assay is a multiplexed assay for the activities of a plurality of lysosomal enzymes in the sample. The compositions and methods can comprise or employ: one or more metal cations effective for precipitating sulfate ions, one or more metal cations effective for precipitating phosphate ions, a maltase glucoamylase inhibitor, a beta-N-acetylhexosaminidase inhibitor, and one or more surfactants.


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