The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 06, 2016

Filed:

Nov. 13, 2013
Applicants:

Bayer Technology Services Gmbh, Leverkusen, DE;

Bayer Materialscience Ag, Leverkusen, DE;

Inventor:

Jorgen Magnus, Düsseldorf, DE;

Assignee:

Covestro Deutschland AG, Leverkusen, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/88 (2006.01); C12P 7/22 (2006.01); C07K 14/245 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01);
U.S. Cl.
CPC ...
C12P 7/22 (2013.01); C07K 14/245 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12Y 401/01061 (2013.01); C12Y 401/0304 (2013.01); C12Y 402/01051 (2013.01); C12Y 504/99005 (2013.01);
Abstract

The invention relates to a method of generating a recombinant host strain for producing phenol, comprising the steps of a) providing a host comprising chorismate, b) transforming the host with a first nucleic acid sequence comprising ubiC (SEQ ID NO: 1) encoding chorismate lyase that converts chorismate to 4-hydroxybenzoate, and c) transforming the host with a second nucleic acid sequence encoding an oxygen-tolerant 4-hydroxybenzoate decarboxylase that converts 4-hydroxybenzoate to phenol, thereby generating a recombinant host that is capable of producing phenol under aerobic conditions, wherein step b) and step c) are carried out simultaneously or sequentially. The invention also provides the recombinant host strain for producing phenol obtainable by the aforementioned method, as well as a method of producing phenol in the recombinant host strain.


Find Patent Forward Citations

Loading…