The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 29, 2016

Filed:

Mar. 15, 2013
Applicant:

The Johns Hopkins University, Baltimore, MD (US);

Inventors:

Sharon Gerecht, Baltimore, MD (US);

Sravanti Kusuma, Baltimore, MD (US);

Assignee:

THE JOHNS HOPKINS UNIVERSITY, Baltimore, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); A61K 38/17 (2006.01); C07K 14/00 (2006.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/069 (2013.01); C12N 5/0691 (2013.01); C12N 5/0692 (2013.01); C12N 2501/15 (2013.01); C12N 2501/165 (2013.01); C12N 2501/17 (2013.01); C12N 2501/42 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/80 (2013.01);
Abstract

A bicellular vascular population derived from human pluripotent stem cells (hPSCs) undergoes morphogenesis and assembly in a synthetic hydrogel. It is shown that hPSCs can be induced to co-differentiate into early vascular cells (EVCs) in a clinically-relevant strategy dependent upon Notch activation. These EVCs mature into ECs and pericytes, and self-organize to form vascular networks in an engineered matrix. Upon in vivo implantation, multicellular human vascular networks are functionally perfused. Thus, a derived bicellular population is exploited for its intrinsic self-assembly capability to create functional microvasculature in a deliverable matrix.


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