The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 29, 2016

Filed:

May. 21, 2013
Applicant:

University of Massachusetts, Boston, MA (US);

Inventors:

Michael P. Czech, Westborough, MA (US);

Mengxi Wang, Worcester, MA (US);

Assignee:

University of Massachusetts, Boston, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 5/077 (2010.01); A61K 45/06 (2006.01); A61K 31/7105 (2006.01); A61K 31/7115 (2006.01); A61K 31/712 (2006.01); A61K 31/7125 (2006.01); A61K 31/713 (2006.01); G01N 33/50 (2006.01); C12N 15/113 (2010.01); A61K 9/00 (2006.01); A61K 9/127 (2006.01); A61K 9/51 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0658 (2013.01); A61K 9/0019 (2013.01); A61K 9/127 (2013.01); A61K 9/51 (2013.01); A61K 31/712 (2013.01); A61K 31/713 (2013.01); A61K 31/7105 (2013.01); A61K 31/7115 (2013.01); A61K 31/7125 (2013.01); A61K 45/06 (2013.01); C12N 15/1137 (2013.01); C12Y 207/11001 (2013.01); G01N 33/5023 (2013.01); G01N 33/5061 (2013.01); G01N 33/5073 (2013.01); C12N 2310/11 (2013.01); C12N 2310/12 (2013.01); C12N 2310/14 (2013.01); C12N 2501/40 (2013.01); C12N 2506/1323 (2013.01); G01N 2333/91205 (2013.01); G01N 2333/91215 (2013.01); G01N 2800/10 (2013.01);
Abstract

Provided herein are methods of inducing differentiation of a mammalian myoblast into a mammalian myocyte that include contacting a mammalian myoblast with an oligonucleotide that decreases Mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of inducing mammalian myoblasts or myocytes to form a myotube that include contacting two or more mammalian myoblasts or two or more mammalian myocytes with an oligonucleotide that decreases Map4k4 mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of identifying a candidate agent useful for inducing muscle formation, and compositions containing an oligonucleotide that decreases Map4k4 mRNA expression in mammalian myoblast or myocyte and one or more additional muscle therapeutic agents and/or muscle-building neutraceuticals.


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