The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 29, 2016

Filed:

Apr. 24, 2009
Applicants:

James Yoo, Winston-Salem, NC (US);

Joel Stitzel, Winston Salem, NC (US);

Anthony Atala, Winston-Salem, NC (US);

George Christ, Winston-Salem, NC (US);

Inventors:

James Yoo, Winston-Salem, NC (US);

Joel Stitzel, Winston Salem, NC (US);

Anthony Atala, Winston-Salem, NC (US);

George Christ, Winston-Salem, NC (US);

Assignee:

Wake Forest University Health Sciences, Winston-Salem, NC (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61F 2/08 (2006.01); C12M 1/42 (2006.01); A61L 27/38 (2006.01); C12M 3/00 (2006.01); C12N 5/077 (2010.01);
U.S. Cl.
CPC ...
C12M 35/04 (2013.01); A61L 27/3826 (2013.01); A61L 27/3895 (2013.01); C12M 21/08 (2013.01); C12N 5/0658 (2013.01);
Abstract

A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.


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