The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 29, 2016
Filed:
Jul. 08, 2014
Applicant:
Msd Oss B.v., Rahway, NJ (US);
Inventors:
Peter Van Der Meijden, Oss, NL;
Gijsbert Willem Karel Van Dedem, Oss, NL;
Michel Hendrikus Maria Eppink, Oss, NL;
Roeland Wilhelmus Wassenaar, Oss, NL;
Assignee:
MSD OSS B.V., Oss, NL;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/14 (2006.01); C07K 1/113 (2006.01); C07K 1/36 (2006.01); C07K 14/62 (2006.01);
U.S. Cl.
CPC ...
C07K 1/14 (2013.01); C07K 1/113 (2013.01); C07K 1/36 (2013.01); C07K 14/62 (2013.01);
Abstract
The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.