The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 22, 2016
Filed:
Jan. 11, 2013
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
James G. Nadeau, Ellicott City, MD (US);
Tobin Hellyer, Westminster, MD (US);
Dolores M. Berger, Baltimore, MD (US);
William Nussbaumer, Baltimore, MD (US);
Robert Rosenstein, Ellicott City, MD (US);
Andrew Kuhn, Baltimore, MD (US);
Sha-Sha Wang, Wellesley, MA (US);
Keith Edward Thornton, Owings Mill, MD (US);
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Abstract
A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte.