The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 22, 2016
Filed:
Nov. 09, 2011
Selection of triploid cho cells and cell lines expressing recombinant polypeptides as high producers
Applicant:
Darja Obrstar, Menges, SI;
Inventor:
Darja Obrstar, Menges, SI;
Assignee:
LEK PHARMACEUTICALS D.D., Ljubljana, SI;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12N 15/67 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/68 (2013.01); C12N 15/67 (2013.01); C12P 21/00 (2013.01);
Abstract
The present invention relates to a method for selecting for cells or cell lines that produce a recombinant protein/polypeptide in high yields, the method allowing for the selection of high producer cells or cell lines in an early phase of cell line development, the method comprising the step of determining the nuclear DNA content of the cells or cell lines, wherein the level of the nuclear DNA content of the cells or cell lines positively correlates with the capacity of the cells or cell lines to produce the recombinant protein/polypeptide.