The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2016

Filed:

Mar. 03, 2011
Applicants:

Richard F. Selden, Lincoln, MA (US);

Eugene Tan, Arlington, MA (US);

Heung Chuan Lam, Newton, MA (US);

Heidi Susanne Giese, Newburyport, MA (US);

Gregory John Kellogg, Cambridge, MA (US);

John A. Wright, Billerica, MA (US);

Inventors:

Richard F. Selden, Lincoln, MA (US);

Eugene Tan, Arlington, MA (US);

Heung Chuan Lam, Newton, MA (US);

Heidi Susanne Giese, Newburyport, MA (US);

Gregory John Kellogg, Cambridge, MA (US);

John A. Wright, Billerica, MA (US);

Assignee:

NetBio, Inc., Waltham, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); G01N 21/64 (2006.01); B01L 3/00 (2006.01); G01N 27/447 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
G01N 21/6486 (2013.01); B01L 3/502753 (2013.01); G01N 21/6452 (2013.01); G01N 27/44726 (2013.01); G01N 27/44782 (2013.01); G01N 27/44791 (2013.01); B01L 7/52 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/10 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0421 (2013.01); G01N 21/6428 (2013.01); Y10T 436/2575 (2015.01);
Abstract

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.


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