The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 15, 2016
Filed:
May. 26, 2011
David Bensimon, Paris, FR;
Vincent Croquette, Antony, FR;
Jean-françois Allemand, Arcueil, FR;
Maria Manosas, Barcelona, ES;
Fang-yuan Ding, Paris, FR;
David Bensimon, Paris, FR;
Vincent Croquette, Antony, FR;
Jean-François Allemand, Arcueil, FR;
Maria Manosas, Barcelona, ES;
Fang-Yuan Ding, Paris, FR;
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), Paris, FR;
ECOLE NORMALE SUPERIEURE, Paris, FR;
UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6), Paris, FR;
Abstract
Described herein is a method for determining a nucleic acid sequence, said method comprising: a) denaturing a double-stranded nucleic acid molecule corresponding to the said nucleic acid sequence; b) hybridizing a single-stranded nucleic acid molecule, the primer, with the said denatured double-stranded nucleic acid molecule; c) applying a tension to the hybridized primer/double-stranded nucleic acid molecule obtained in b); d) incubating the hybridized primer/double-stranded nucleic acid molecule obtained in b) with a polymerase in conditions which will lead to at least one pause in replication; and e) determining a position of the said pause in replication with respect to one end of the double-stranded nucleic acid.