The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 15, 2016
Filed:
Jan. 13, 2011
Christof Von Kalle, Freiburg, DE;
Manfred Schmidt, St. Leon-Rot, DE;
Richard Gabriel, Schriesheim, DE;
Ali Nowrouzi, Heidelberg, DE;
Anne Arens, Schwetzingen, DE;
Angelo Lombardo, Milan, IT;
Luigi Naldini, Milan, IT;
Christof Von Kalle, Freiburg, DE;
Manfred Schmidt, St. Leon-Rot, DE;
Richard Gabriel, Schriesheim, DE;
Ali Nowrouzi, Heidelberg, DE;
Anne Arens, Schwetzingen, DE;
Angelo Lombardo, Milan, IT;
Luigi Naldini, Milan, IT;
Deutsches Krebsforschungszentrum, Heidelberg, DE;
Ospedale San Raffaele SRL, Milan, IT;
Abstract
The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease.