The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 15, 2016
Filed:
Sep. 21, 2011
YE Du, Shenzhen, CN;
Meiru Zhao, Shenzhen, CN;
Ying Chen, Shenzhen, CN;
Jinghua Wu, Shenzhen, CN;
Geng Tian, Shenzhen, CN;
Jun Wang, Shenzhen, CN;
Ye Du, Shenzhen, CN;
Meiru Zhao, Shenzhen, CN;
Ying Chen, Shenzhen, CN;
Jinghua Wu, Shenzhen, CN;
Geng Tian, Shenzhen, CN;
Jun Wang, Shenzhen, CN;
BGI TECH SOLUTIONS CO., LTD., Shenzhen, CN;
Abstract
A DNA library, and a preparing method thereof, a method of determining DNA sequence information, an apparatus and a kit for detecting SNPs, and a method for genotyping may be provided. The method for preparing the DNA library may comprise the steps of: digesting a genomic DNA sample using a restriction endonuclease to obtain a digested product, wherein the restriction endonuclease comprises at least one selected from the group consisting of Mbo II and Tsp 45I; separating the digested product to obtain DNA fragments having a length of 100 bp to 1,000 bp; end-repairing the DNA fragments to obtain an end-repaired DNA fragments; adding a base A to the end of the end-repaired DNA fragments to obtain DNA fragments having a terminal base A; and ligating the DNA fragments having the terminal base A with sequencing adaptors to obtain the DNA library.