The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 15, 2016
Filed:
Sep. 12, 2012
Applicants:
Mattias Collin, Lund, SE;
Maria Allhorn, Lund, SE;
Jonathan Sjögren, Lund, SE;
Inventors:
Assignee:
Genovis AB, Lund, SE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C12N 9/24 (2006.01); G01N 33/569 (2006.01); C12P 21/00 (2006.01); C12Q 1/34 (2006.01); C12Q 1/37 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2405 (2013.01); C12N 9/2402 (2013.01); C12P 21/005 (2013.01); C12Q 1/34 (2013.01); C12Q 1/37 (2013.01); C12Y 302/01096 (2013.01); G01N 33/56944 (2013.01); G01N 33/6854 (2013.01); C07K 2317/41 (2013.01); G01N 2469/20 (2013.01);
Abstract
The invention provides an endoglycosidase, referred to as EndoS49 and having the amino acid sequence of SEQ ID NO: 1. EndoS49 was isolated fromstrain NZ131 and is a homolog of EndoS. EndoS49 has specific endoglycosidase activity on native IgG and cleaves a larger variety of Fc glycans than EndoS. A mutant thereof where the glutamic acid at position 186 of SEQ ID NO: 1 was substituted was produced: said mutant lacks endoglycosidase activity but is capable of binding to IgG. Methods using EndoS49, deletions thereof and said mutant, especially for assessing glycosylation of IgG or for isolating IgG are disclosed.