The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2016

Filed:

Oct. 18, 2012
Applicants:

Sarl Endocells, Paris, FR;

Centre National DE LA Recherche Scientifique (Cnrs), Paris, FR;

Institut National DE LA Sante ET DE LA Recherche Medicale (Inserm), Paris, FR;

Inventors:

Paul Czernichow, Paris, FR;

Philippe Ravassard, Paris, FR;

Raphael Scharfmann, Paris, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 48/00 (2006.01); C12N 5/071 (2010.01); A01K 67/027 (2006.01); C12N 5/09 (2010.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0676 (2013.01); A01K 67/0271 (2013.01); C12N 5/0677 (2013.01); C12N 5/0693 (2013.01); G01N 33/5023 (2013.01); G01N 33/56966 (2013.01); A01K 2207/12 (2013.01); A01K 2227/105 (2013.01); A01K 2267/025 (2013.01); A01K 2267/0362 (2013.01); C12N 2501/33 (2013.01); C12N 2501/734 (2013.01); C12N 2503/00 (2013.01); C12N 2510/04 (2013.01);
Abstract

The present invention relates to a method for preparing commercial scale quantities of human functional beta cells and to the establishment of cell lines from non-foetal pancreatic tissues. It also relates to a method of diagnosis using beta cell tumors or cells derived thereof. The method comprises sub-transplantation procedure to enrich the graft in proliferating beta cells, allowing to generate human Beta cell lines. Such lines express, produce and secrete insulin upon glucose stimulation. They have a gene expression profile that resembles to adult beta cells. In addition, the human beta cell lines are able to normalize glycemia of diabetic mice when transplanted, demonstrating their insulin secretion capabilities.


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