The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2016

Filed:

Jun. 22, 2012
Applicants:

Yasuo Yoshimoto, Tokyo, JP;

Kentaro Kamimae, Tokyo, JP;

Yuki Tanabe, Tokyo, JP;

Taku Oguni, Ichihara, JP;

Takashi Inoue, Ichihara, JP;

Tsutomu Mochizuki, Ichihara, JP;

Makoto Hirama, Ichihara, JP;

Teruo Fujii, Tokyo, JP;

Hiroshi Kimura, Tokyo, JP;

Hideto Tozawa, Tokyo, JP;

Inventors:

Yasuo Yoshimoto, Tokyo, JP;

Kentaro Kamimae, Tokyo, JP;

Yuki Tanabe, Tokyo, JP;

Taku Oguni, Ichihara, JP;

Takashi Inoue, Ichihara, JP;

Tsutomu Mochizuki, Ichihara, JP;

Makoto Hirama, Ichihara, JP;

Teruo Fujii, Tokyo, JP;

Hiroshi Kimura, Tokyo, JP;

Hideto Tozawa, Tokyo, JP;

Assignees:

KISCO LTD., Osaka, JP;

DAISANKASEI CO., LTD., Tokyo, JP;

The University of Tokyo, Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B05D 1/00 (2006.01); C12M 1/12 (2006.01); C12N 5/00 (2006.01); C12M 1/00 (2006.01);
U.S. Cl.
CPC ...
B05D 1/60 (2013.01); C12M 23/20 (2013.01); C12M 25/02 (2013.01); C12N 5/0068 (2013.01); C12N 2533/30 (2013.01);
Abstract

Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH)—NHmoiety (n is an integer of 1-10 inclusive.) or H, with at least one of R1 and R2 being a —(CH)—NHmoiety. Moreover, l and m are positive integers expressing polymerization degree).


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