The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 2016

Filed:

Jan. 20, 2014
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Anthony J. Makarewicz, Livermore, CA (US);

Amy L. Hiddessen, Dublin, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01F 3/08 (2006.01); B01F 13/00 (2006.01); B01L 3/02 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 15/14 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
B01F 3/0807 (2013.01); B01F 13/0062 (2013.01); B01L 3/0241 (2013.01); B01L 3/502784 (2013.01); B01L 7/525 (2013.01); G01N 15/1404 (2013.01); G01N 15/1434 (2013.01); G01N 15/1459 (2013.01); G01N 15/1463 (2013.01); G01N 15/1484 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/088 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); G01N 15/1436 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1415 (2013.01);
Abstract

Method of detection for droplet-based assays. In an exemplary method, an open end of a channel network may be placed into an emulsion. Droplets of the emulsion may be driven along a flow path from the open end, through a confluence region where a dilution fluid is introduced into the flow path from at least one dilution inlet channel to increase an average distance between droplets, and through an examination region disposed downstream of the confluence region. Light may be detected from the examination region as droplets pass through. The channel network may include a droplet inlet channel that meets the at least one dilution inlet channel at the confluence region. The droplet inlet channel may form a tapered region that is sized such that droplets leave the tapered region in single file.


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