The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 15, 2016
Filed:
Apr. 26, 2012
Kumiko Saeki, Tokyo, JP;
Akira Yuo, Tokyo, JP;
Miwako Nishio, Tokyo, JP;
Masako Kawasaki, Tokyo, JP;
Koichi Saeki, Ibaraki, JP;
Mamoru Hasegawa, Ibaraki, JP;
Kumiko Saeki, Tokyo, JP;
Akira Yuo, Tokyo, JP;
Miwako Nishio, Tokyo, JP;
Masako Kawasaki, Tokyo, JP;
Koichi Saeki, Ibaraki, JP;
Mamoru Hasegawa, Ibaraki, JP;
ID Pharma Co., Ltd., Ibaraki, JP;
National Center for Global Health and Medicine, Tokyo, JP;
Abstract
Provided are a method of producing brown adipocytes from pluripotent stem cells, a method of producing cell aggregates as an intermediate product thereof, pluripotent stem cell-derived cell aggregates and pluripotent stem cell-derived brown adipocytes produced by these methods, and cell therapy using the pluripotent stem cell-derived brown adipocytes. In the method of producing brown adipocytes from pluripotent stem cells, cell aggregates are produced from pluripotent stem cells by a method including the step (A), and brown adipocytes are prepared from the cell aggregates by a method including the step (B). The step (A) is a step of producing cell aggregates by non-adhesive culture of pluripotent stem cells in serum-free environment in the presence of a hematopoietic cytokine, and the step (B) is a step of producing brown adipocytes by adhesion culture of the cell aggregates in the presence of a hematopoietic cytokine.