The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 08, 2016

Filed:

May. 17, 2013
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventor:

Christopher Belisle, Walnut Creek, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); H01J 49/00 (2006.01); G01N 30/06 (2006.01); G01N 33/68 (2006.01); H01J 49/26 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44721 (2013.01); G01N 30/06 (2013.01); G01N 33/6848 (2013.01); H01J 49/0036 (2013.01); H01J 49/26 (2013.01);
Abstract

Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.


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