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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 9487809 B1

PDFFull Text
Date of Patent:
Nov. 08, 2016

Filed:

Nov. 28, 2012

Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase

Applicant:

Genentech, Inc., South San Francisco, CA (US);

Inventors:

Meixia Zhou, Foster City, CA (US);

Bradley Richard Snedecor, Portola Valley, CA (US);

Chi Kin Domingos Ng, San Francisco, CA (US);

Amy Shen, San Mateo, CA (US);

Assignee:

Genentech, Inc., South San Francisco, CA (US);

Attorney:

Morrison & Foerster LLP

Primary Examiner:

Jane Zara

Int. Cl.
CPC ...
C12P 21/00 (2006.01); A61K 48/00 (2006.01); C12N 15/85 (2006.01); C12N 15/113 (2010.01); C12N 15/11 (2006.01); C12N 1/38 (2006.01);
U.S. Cl.
CPC ...
C12P 21/00 (2013.01); C12N 1/38 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 15/85 (2013.01); C12N 2310/14 (2013.01); C12N 2320/00 (2013.01);
Abstract

The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

Find Patent Forward Citations

Zhou, Meixia. (2016). Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase (U.S. Patent No. 9487809). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/9487809

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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