The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 01, 2016
Filed:
Mar. 21, 2014
Applicant:
Nestec S.a., Vevey, CH;
Inventors:
Hua Gong, San Diego, CA (US);
Shui Long Wang, San Diego, CA (US);
Sharat Singh, Rancho Santa Fe, CA (US);
Assignee:
Nestec S.A., Vevey, CH;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 31/00 (2006.01); G01N 33/573 (2006.01); G01N 33/68 (2006.01); G01N 33/74 (2006.01);
U.S. Cl.
CPC ...
G01N 33/573 (2013.01); G01N 33/6893 (2013.01); G01N 33/74 (2013.01); G01N 2800/065 (2013.01); G01N 2800/56 (2013.01); Y10S 436/809 (2013.01); Y10T 436/147777 (2015.01);
Abstract
The invention provides an ELISA assay for the determination of serum mast cell β-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of β-tryptase, histamine and/or prostaglandin E.