The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 01, 2016

Filed:

Jun. 19, 2015
Applicant:

Applied Biosystems, Llc, Carlsbad, CA (US);

Inventors:

Jaiprakash Shewale, Santa Clara, CA (US);

Manohar Furtado, San Ramon, CA (US);

Pius Brzoska, Woodside, CA (US);

Maura Barbisin, San Mateo, CA (US);

Rixun Fang, Menlo Park, CA (US);

Michael Malicdem, Santa Clara, CA (US);

Cristin O'Shea, Mountain View, CA (US);

Assignee:

APPLIED BIOSYSTEMS, LLC, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6879 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/6888 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01);
Abstract

The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.


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